MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Aoki S, Jikiba A, Takeda K, Kimura E
Article Title: A zinc(II) complex-conjugated polymer for selective recognition and separation of phosphates.
Publication date: 2004
Journal: Journal of Physical Organic Chemistry
Volume: 17
Issue: (6-7)
Page numbers: 489-497.
DOI: 10.1002/poc.773

Abstract: Anew polymer, Zn2+-cyclen complex-conjugated polymer 4 (cyclen = 1,4,7, 10-tetraazacyclododecane), was synthesized by copolymerization of a Zn2+-(4-vinyl)benzylcyclen complex (3, ZnL2) with ethylene glycol dimethacrylate (EGDMA). The contents of the zinc(11) ions in Zn2+-cyclen polymer 4 were determined as 36-42 mumol g(-1), utilizing a Zn2+- selective fluorophore, dansylamidoethylcyclen 10 (L-3). It was found that 31% of deoxyadenosine 5'-monophosphate (5'-dAMP) (50 mum) is adsorbed on 4 (corresponding to 50 pm) in 20 mm HEPES (pH 7.0) with I = 0.1 (NaNO3) at 25 degreesC. The apparent complexation constants for the 1:I complex of ZnL2 on 4 with 5-dAMP and 4-NPP, logK(app)(ZnL-S2-) (S2- denotes a phosphomonoester dianion), at pH 7.0 and 25 degreesC were determined as 4.1 +/- 0.1, which was larger than that (3.5) for the 1: 1 complex of 3 (ZnL2) with 5'-dAMP in homogeneous aqueous solution at pH 7.0 and 25 degreesC. Deoxyadenosine (dA) and adenosine 3',5'-cyclic- monophosphate (3,5'-cAMP), a cyclic phosphodiester monoanion, were negligibly adsorbed on Zn2+-cyclen polymer 4, implying that 4 interacts selectively with phosphomonoester dianions. For reference, Zn2+ free polymer 8 (prepared from 4) and control polymer 9 (prepared by copolymerization of styrene with EGDMA) hardly adsorbed 5'-dAMP, dA and 3,5'-cAMP. High- performance liquid chromatography (HPLC) of mononucleotides such as 5'-dAMP, cytidine 5'-monophosphate (5-CMP), guanosine 5'-monophosphate (5'-GMP), and thymidine 5'-monophosphate (5'- dTMP) was achieved using 4 as a stationary phase and continuous gradient elution with 10:90 MeOH-1 mm HEPES (pH 7.0) and 10:90 MeOH-1 mm HEPES + 10 mm (NH4)(2)HPO4 (pH 7.0). As expected, the retention times of mononucleotides were larger than those of the corresponding nucleosides, owing to the Zn2+-phosphate interactions. These results indicated that Zn2+-cyclen- conjugated polymer 4 affords a strong methodology to detect and separate biologically important phosphates. Copyright (C) 2004 John Wiley Sons, Ltd


  Krypton periodic table mug  Scientist peptide mug  I shop perodically customisable tote bag

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner