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Authors: Wandelt B, Mielniczak A, Cywinski P
Article Title: Monitoring of cAMP-imprinted polymer by fluorescence spectroscopy.
Publication date: 2004
Journal: Biosensors and Bioelectronics
Volume: 20
Issue: (6)
Page numbers: 1031-1039.
DOI: 10.1016/j.bios.2004.07.035
Alternative URL: http://www.sciencedirect.com/science/article/B6TFC-4DT2JSX-1/2/3ef3de93b0755979095f3c2b1157735e
Abstract: Conventional functional monomers together with fluorescent monomer, trans-4-[p-(N,N-dimethylamino)styryl]-N-vinylbenzylpyridinium chloride (vb-DMASP), were copolymerised in the presence of a target molecule, nucleotide-cAMP that acted as a molecular template. The polymer was copolymerised in thin-layer films. After removal of the template the functionalised cavities that exist in the fluorescent material are able to specifically bind the template. Subsequent adsorption of the template-cAMP causes quenching of fluorescence of the polymer. The specific photochemical processes accompanying the template adsorption are discussed further. The imprinted polymers monitored by both steady-state and time-resolved fluorescence techniques show specificity and selectivity of binding of the template on the imprinted functionalised cavities
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