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Reference type: Journal
Authors: Lei JD, Tong AJ
Article Title: Preparation of Z-l-Phe-OH-NBD imprinted microchannel and its molecular recognition study.
Publication date: 2005
Journal: Spectrochimica Acta Part A-Molecular and Biomolecular Spctroscopy
Volume: 61
Issue: (6)
Page numbers: 1029-1033.
DOI: 10.1016/j.saa.2004.06.001
Alternative URL: http://www.sciencedirect.com/science/article/B6VNG-4DTSN11-1/2/3542bccbd1ce657c3b09f05643f86f72

Abstract: An integrated microchip was presented for selective recognition of Z-l-Phe-OH-NBD, using molecular imprinting technique. Molecularly imprinted polymer (MIP) were prepared by copolymerization in the presence of template molecule Z-l-Phe-OH-NBD, in which methacrylic acid and 4-vinylpyridine were used as functional monomers and ethylene dimethacrylate used as crosslinker. Imprinted polymer particles were introduced into a microchannel fabricated with a new material i.e. poly(methylvinylsiloxane) by simply rapid prototyping method. Imprinted effects were evaluated by laser-induced fluorescence (LIF) detection where the results indicated that good selective recognition for Z-l-Phe-OH-NBD in the imprinted polymer was obtained; the adsorption percentage of Z-l-Phe-OH-NBD was 61%. In contrast to conventional molecular imprinting analysis, integration shortened overall analysis time from 4 h to 10 min
Template and target information: Z-l-Phe-OH-NBD
Author keywords: Microchannel, Molecularly imprinted polymers, laser-induced fluorescence, selective recognition


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