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Abstract: Though acrylamide polymers are widely used in electrophoretic and chromatographic techniques, many research works aim at optimizing these media. As polyacrylamide presents some drawbacks, new acrylic monomers with particular properties are proposed. In this paper, we described some approaches for obtaining polymers which are more porous, more hydrophilic and more resistant in alkaline solutions. Moreover, we also detail the synthesis of particular functionalized monomers, explaining the concept of 'polymer design' applicable to the techniques of protein separation.