MIPdatabase
Authors: Uragami T, Jige M, Miyata T
Publication date: 2004
Article title: Preparation of glycoprotein-responsive hydrogels by biomolecular imprinting and effect of gel structure on their shrinking behavior.
Page numbers: 675
Alternative URL: http://www.jstage.jst.go.jp/article/spsjppj/51m/0/51m_675/_article
Proceedings title: Polymer Preprints, Japan
Volume number: 53
Abstract: a-Fetoprotein (AFP) which is a glycoprotein is widely used as a diagnostic tumor-specific marker. In this study, novel stimuli-responsive hydrogels that underwent swelling changes in response to AFP were prepared by biomolecular imprinting using lectin and antibody as ligands for AFP. In the biomolecular imprinting, vinyl-antibody was copolymerized with acrylamide and methylenebisacrylamide with forming a lectin-AFP-antibody complex in the presence of polymerized lectin, and then AFP as a print biomolecule was removed from the resultant hydrogel. The AFP-imprinted hydrogel was shrunken in the phosphate buffer solution containing AFP, but nonimprinted hydrogel was a little swollen. The AFP-responsive shrinking of the AFP-imprinted hydrogel is due to the fact that the formation of a lectin-AFP-antibody complex in the hydrogel results in increasing cross-linking density. These imply that the network of AFP-imprinted hydrogel momorized AFP.
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