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Authors: Uragami T, Jige M, Miyata T
Publication date: 2002
Article title: Synthesis of glycoprotein-sensitive hydrogels with multi-ligands by biomolecular imprinting and their glycoprotein-responsiveness.
Page numbers: 1841
Alternative URL: http://www.jstage.jst.go.jp/article/spsjppj/51/0/51_1841/_article
Proceedings title: Polymer Preprints, Japan
Volume number: 51
Abstract: a-Fetoprotein (AFP) which is a glycoprotein is widely used as a diagnostic tumor-specific marker. In this study, novel stimuli-responsive hydrogels that underwent swelling changes in response to AFP were prepared by biomolecular imprinting using lectin and antibody as ligands for AFP. In the biomolecular imprinting, vinyl-antibody was copolymerized with acrylamide and methylenebisacrylamide with forming a lectin-AFP-antibody complex in the presence of polymerized lectin, and the AFP as a print biomolecule was removed from the resultant hydrogel. The AFP-imprinted hydrogel was shrunken in the phosphate buffer solution containing AFP, but nonimprinted hydrogel was a little swollen. The AFP-responsive shrinking of the AFP-imprinted hydrogel is due to the fact that the formation of a lectin-AFP-antibody complex in the hydrogel results in increasing cross-linking density.
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