Abstract: The molecularly imprinted solid-phase extraction (SPE) cartridge of chloramphenicol (CAP) was prepared by adopting molecular imprinting technique, and its absorption ability as well as desorption conditions was investigated, on the basis of which the working conditions of SPE cartridge, including the solvent for CAP, the rinse and elution conditions, were ascertained. The results indicate that, compared to the traditional CIS cartridges, the molecularly imprinted solid-phase extraction cartridge is of great advantages, that is, it can use methanol-water solution with high methanol content as the rinse solution, acetonitrile-water solution with 40% (volume fraction) acetonitrile as the elution solution, and it is of a greater cartridge capacity than CIS cartridges. All these advantages make it possible to apply the SPE cartridge to the CAP detection of actual bio-products, thus resolving the problems existing in the CAP residue detection at present
Template and target information: chloramphenicol, CAP