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Reference type: Journal
Authors: Flores A, Cunliffe D, Whitcombe MJ, Vulfson EN
Article Title: Imprinted polymers prepared by aqueous suspension polymerization.
Publication date: 2000
Journal: Journal of Applied Polymer Science
Volume: 77
Issue: (8)
Page numbers: 1841-1850.
DOI: 10.1002/1097-4628(20000822)77:8<1841::AID-APP22>3.0.CO;2-P

Abstract: The feasibility of preparing cholesterol-imprinted polymers by aqueous suspension polymerization was investigated by the preparation of ethyleneglycol dimethacrylate and divinylbenzene-based beads imprinted using cholesteryl(4- vinyl)-phenyl carbonate as the template. A low volatility porogen to replace a 4:1 hexane/ toluene mixture was selected on the basis of solubility parameters and consisted of dioctyl phthalate/n-decane 77:23 v/v. Beads were prepared using a range of porogen contents with the best results obtained at 5.5-6.5 mL /5 g of monomer. Uptake of cholesterol by suspension polymers was broadly similar to that of the corresponding "bulk" polymers, but suffered from higher nonspecific binding. (C) 2000 John Wiley & Sons, Inc

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