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Abstract: Uniformly sized molecularly imprinted polymers (MIPs) for (S)- nilvadipine have been prepared by a multistep swelling and polymerization method using methacrylic acid, 2- (trifluoromethyl)acrylic acid, 2-vinylpyridine, or 4- vinylpyridine (4-VPY) as a functional monomer and ethylene glycol dimethacrylate (EDMA) as a cross-linker. The chiral recognition abilities of the MIPs for nilvadipine and other dihydropyridine calcium antagonists were evaluated using a mixture of sodium phosphate buffer (or water) and acetonitrile or only acetonitrile as the mobile phase. The (S)-nilvadipine- imprinted 4-VPY-co-EDMA polymers gave the highest resolution for nilvadipine among the MIPs prepared. In addition, the enantio-separation of nilvadipine was attained using the (S)nilvadipine-imprinted EDMA polymers, without use of a functional monomer. H-1 NMR and molecular modeling studies suggested a one-to-one hydrogen-bonding-based complex formation of (S)-nilvadipine with 4-VPY in chloroform. These results reveal that the (S)-nilvadipine-imprinted EDMA polymers could recognize the template molecule by its molecular shape, and that in addition to this recognition, hydrophobic and hydrogen- bonding interactions seems to play important roles in the retention and chiral recognition of nilvadipine on the 4-VPY- co-EDMA polymers in hydroorganic mobile phases. By optimizing chromatographic conditions such as column temperature and flow rate, the baseline separation of nilvadipine enantiomers was attained with a short analysis time and with a column efficiency comparable to commercially available chiral stationary phases based on a protein, such as ovomucoid or alpha(1)-acid glycoprotein