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Reference type: Journal
Authors: Yoshikawa M, Kawamura K, Ejima A, Aoki T, Sakurai S, Hayashi K, Watanabe K
Article Title: Green polymers from Geobacillus thermodenitrificans DSM465 - Candidates for molecularly imprinted materials.
Publication date: 2006
Journal: Macromolecular Bioscience
Volume: 6
Issue: (3)
Page numbers: 210-215.
DOI: 10.1002/mabi.200500187

Abstract: Summary: Novel molecular recognition materials were prepared from water soluble proteins from thermophile G. thermodenitrificans DSM465 by an alternative molecular imprinting method. Water soluble proteins from G. thermodenitrificans DSM465 were converted into the molecularly imprinted materials by adopting 9-EA as a print molecule. The molecularly imprinted protein membranes recognized As in preference to Gs. The adsorption isotherms led to the conclusion that molecular recognition sites toward As were constructed by the presence of 9-EA during the membrane preparation process. The affinity constant between As and the molecular recognition site thus constructed was determined to be 1.75105 mol-1 dm3. The results obtained in the present study suggest that water soluble proteins from G. thermodenitrificans DSM465 is one of environmentally-friendly "green" polymers to be converted into molecular recognition materials by applying an alternative molecular imprinting method.AsGs9-EAAs9-EAGs9-EAAsGs
Template and target information: 9-ethyladenine, 9-EA, adenine
Author keywords: adenosine, adsorption, 9-ethyladenine, Geobacillus thermodinitrificans DSM465, guanosine, molecular imprinting, molecular recognition, proteins


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