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Reference type: Journal
Authors: Minoura N, Ogiso M, Shmizu T
Article Title: Detection of double-stranded DNA by utilizing a recognition cavity in molecular imprinting polymer.
Publication date: 2005
Journal: Polymer Preprints, Japan
Volume: 54
Issue: (2)
Page numbers: 4749-4750.

Abstract: In order to develop a simple and inexpensive detection method, we prepared a molecularly imprinted polymer (MIP) gel for recognizing a specific double-stranded DNA (dsDNA) target sequence in MIP gel electrophoresis. The detection of target dsDNA in this MIP-electrophoresis system was based on the decreasing of migration distance by the capture effect of imprinted cavities. We confirmed that the migration of a target dsDNA in the MIP gel was smaller than that in the polyacrylamide gel (non-imprinted polymer gel). Using this phenomenon, a target dsDNA in a DNA-mixed sample could be detected
Template and target information: DNA, dsDNA
Author keywords: 2-vinyl-4,6- diamino-1,3,5-triazine (VDAT), double-stranded DNA, electrophoresis, molecular recognition, molecularly imprinted polymer


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