Abstract: A novel and simple chemiluminescence (CL) method has been developed and validated for determination of metformin. This method is based on hydroxyl radical chemiluminescence-the hydroxyl radical generated by reaction of Cu(II) and hydrogen peroxide oxidizes rhodamine B (RhB) to produce weak CL which can be enhanced by metformin. At the same time, metformin molecularly imprinted polymer (MIP) was synthesized. After enrichment based on the selectivity of metformin-MIP, the CL method was successfully applied to the determination of metformin in human serum. The linear range was from 1.0 x 10-8 to 1.0 x 10-6 g mL-1 and the detection limit was 4 x 10-9 g mL-1. The relative standard deviation at 2.0 x 10-7 g mL-1 by use of MIP was 3.67% (n=7).
Template and target information: metformin
Author keywords: flow injection, chemiluminescence, hydroxyl radical, metformin, molecularly imprinted polymer