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Reference type: Journal
Authors: He C, Zhang ZJ, He DY, Xiong Y
Article Title: Chemiluminescence determination of metformin based on hydroxyl radical reaction and molecularly imprinted polymer on-line enrichment.
Publication date: 2006
Journal: Analytical and Bioanalytical Chemistry
Volume: 385
Issue: (1)
Page numbers: 128-133.
DOI: 10.1007/s00216-006-0378-5

Abstract: A novel and simple chemiluminescence (CL) method has been developed and validated for determination of metformin. This method is based on hydroxyl radical chemiluminescence-the hydroxyl radical generated by reaction of Cu(II) and hydrogen peroxide oxidizes rhodamine B (RhB) to produce weak CL which can be enhanced by metformin. At the same time, metformin molecularly imprinted polymer (MIP) was synthesized. After enrichment based on the selectivity of metformin-MIP, the CL method was successfully applied to the determination of metformin in human serum. The linear range was from 1.0 x 10-8 to 1.0 x 10-6 g mL-1 and the detection limit was 4 x 10-9 g mL-1. The relative standard deviation at 2.0 x 10-7 g mL-1 by use of MIP was 3.67% (n=7).
Template and target information: metformin
Author keywords: flow injection, chemiluminescence, hydroxyl radical, metformin, molecularly imprinted polymer

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