MIPs logo MIPdatabase      MIP2020 Conference logo Use this space
Custom Search
Reference type: Journal
Authors: Ou JJ, Kong L, Pan CS, Su XY, Lei XY, Zou HF
Article Title: Determination of dl-tetrahydropalmatine in Corydalis yanhusuo by l-tetrahydropalmatine imprinted monolithic column coupling with reversed-phase high performance liquid chromatography.
Publication date: 2006
Journal: Journal of Chromatography A
Volume: 1117
Issue: (2)
Page numbers: 163-169.
DOI: 10.1016/j.chroma.2006.03.084
Alternative URL: http://www.sciencedirect.com/science/article/B6TG8-4JRKCYN-6/2/c338265f3cea4d72f8a22a08d383e68c

Abstract: A method for direct determination of dl-tetrahydropalmatine (dl-THP) in Corydalis yanhusuo, a traditional Chinese herb, by l-THP imprinted monolithic precolumn on-line/off-line coupling with reversed-phase high performance liquid chromatography (RP-HPLC) was developed. The l-THP imprinted monolithic column has been prepared by in situ polymerization using methacrylic acid (MAA) and ethylene dimethacrylate (EDMA) as functional monomer and cross-linker, respectively. With the optimization of chromatographic conditions, such as mobile phase composition, flow rate, column temperature and sample loading, for the separation of enantiomer, dl-THP was base-line separated on the MIP. The imprinted monolithic column was used as a precolumn for fractionation of the C. yanhusuo extract. Both the non-retained and retained fractions were separated by RP-HPLC. Meanwhile, the d-THP and l-THP can be detected in the non-retained and retained fractions, respectively. Additionally, direct determination of l-THP using molecularly imprinted monolith on-line coupling with a reversed-phase column was acquired
Template and target information: l-tetrahydropalmatine, l-THP
Author keywords: molecularly imprinted polymer, monolithic column, Tetrahydropalmatine, chiral separation, on-line, Solid-phase extraction, Corydalis yanhusuo


  Molybdenum periodic table mug  Scientist peptide mug  I shop perodically customisable tote bag

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner