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Reference type: Journal
Authors: Karasová G, Lehotay J, Klodzinska E, Gadzala-Kopciuch R, Buszewski B
Article Title: Comparison of several extraction methods for the isolation of benzoic acid derivatives from Melissa officinalis.
Publication date: 2006
Journal: Journal of Liquid Chromatography & Related Technologies
Volume: 29
Issue: (11)
Page numbers: 1633-1644.
DOI: 10.1080/10826070600678365
Alternative URL: http://www.informaworld.com/10.1080/10826070600678365

Abstract: Several extraction techniques, such as Soxhlet extraction, solid phase extraction using molecularly imprinted polymer, matrix solid phase dispersion, and supercritical fluid extraction were evaluated for the isolation and purification of phenolic compounds, e. g., benzoic acids from natural samples of Melissa officinalis. The extracts of benzoic acids were analyzed by high performance liquid chromatography (HPLC) in reversed phase modus (C-18 column) and under gradient elution (acetonitrile/0.074 mol/L formic acid). The results showed that the recovery rates of gallic acid, p-hydroxybenzoic acid, protocatechuic acid, gentisic acid, vanillic acid, and syringic acid from biological materials by MSPD was equivalent with and, in fact, higher than that of conventional extraction methods
Template and target information: gallic acid, p-hydroxybenzoic acid, protocatechuic acid, gentisic acid, vanillic acid, syringic acid
Author keywords: benzoic acid derivatives, Melissa officinalis, Soxhlet extraction, solid phase extraction using molecularly imprinted polymer-MISPE, Matrix solid phase dispersion-MSPD, supercritical fluid extraction-SFE


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