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Reference type: Journal
Authors: Theodoridis G, Lasáková M, Skeríková V, Tegou A, Giantsiou N, Jandera P
Article Title: Molecular imprinting of natural flavonoid antioxidants: Application in solid-phase extraction for the sample pretreatment of natural products prior to HPLC analysis.
Publication date: 2006
Journal: Journal of Separation Science
Volume: 29
Issue: (15)
Page numbers: 2310-2321.
DOI: 10.1002/jssc.200500492

Abstract: As shown in the past years, SPE based on molecularly imprinted polymers (MIPs) may provide significant enhancement of selectivity in sample preparation and analyte preconcentration. The objective of this work was the fabrication of MIPs for the specific adsorption of rutin and quercetin. The two flavonoids were used as the template molecules for the preparation of MIP phases in a self-assembly (noncovalent) approach. The produced MIPs were validated with regard to the imprinting efficiency as media for LC and SPE. The retention behavior of several flavonoid compounds was studied using as stationary phases imprinted, control nonimprinted polymers, and commercial silica-based materials. MIPs were applied as materials for the selective SPE and preconcentration of the flavonoids from white and red wine, orange juice, and tea. The collected fractions were analyzed by high-pressure LC. MIP-SPE facilitated specific analyte isolation and effective sample clean-up. The results show that molecularly imprinted SPE can be a useful tool for the simple, selective, and cost-effective pretreatment of samples containing natural antioxidants
Template and target information: rutin, quercetin
Author keywords: flavonoids, Molecularly imprinted polymers, quercetin, Rutin, Solid-phase extraction


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