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Reference type: Journal
Authors: Altschuh D, Oncul S, Demchenko AP
Article Title: Fluorescence sensing of intermolecular interactions and development of direct molecular biosensors.
Publication date: 2006
Journal: Journal of Molecular Recognition
Volume: 19
Issue: (6)
Page numbers: 459-477.
DOI: 10.1002/jmr.807

Abstract: Molecular biosensors are devices of molecular size that are designed for sensing different analytes on the basis of biospecific recognition. They should provide two coupled functions-the recognition (specific binding) of the target and the transduction of information about the recognition event into a measurable signal. The present review highlights the achievements and prospects in design and operation of molecular biosensors for which the transduction mechanism is based on fluorescence. We focus on the general strategy of fluorescent molecular sensing, construction of sensor elements, based on natural and designed biopolymers (proteins and nucleic acids). Particular attention is given to the coupling of sensing elements with fluorescent reporter dyes and to the methods for producing efficient fluorescence responses. Copyright 2006 John Wiley & Sons, Ltd
Template and target information: Review - fluorescence biosensors
Author keywords: molecular recognition, target detection, Biosensors, fluorescent dyes, fluorescent labeled proteins, direct fluorescence sensing, molecular biosensors, signal transduction


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