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Abstract: Protein recognition by synthetic materials is of great interest in biotechnology and nanotechnology. Here we report the immobilization of metal complexes on polymer matrices for the preparation of SPR sensors for proteins by molecular imprinting. Vinyl-modified metal complexes were copolymerized in the presence of RNase A as a template protein on the Au-SAM chip by radical polymerization with methylenebisacrylamide. After the template protein was washed out, the binding affinities of the proteins on the polymer surface were studied by SPR. The SPR sensor chip imprinted with RNase A showed higher affinity and selectivity for the template protein. Furthermore, it was possible to regulate the binding affinities of proteins by the removal or exchange of metals in the imprinted polymers. Molecular recognition of proteins by using metalloporphyrin (Zn(TCPP)) as a functional monomer is also discussed
Template and target information: protein, RNase A
Author keywords: metal complex, molecular imprinting, protein, SPR