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Abstract: In this study, we prepared two types of DNA-responsive gels that can swell or shrink in response to a target DNA. DNA-cross-linked gels were synthesized by introducing duplex DNAs as cross-linking points into a poly(acrylamide) (PAAm) gel. DNA-imprinted gels were prepared by molecular imprinting using single stranded DNAs as ligands for a target DNA. In a buffer solution containing a target DNA, the DNA-cross-linked gels swelled remarkably and the DNA-imprinted gels shrank gradually. The DNA-responsive swelling of the DNA-cross-linked gels was due to decreasing cross-linking density of the gels by complex exchange of a target DNA and a mismatch DNA. The DNA-responsive shrinking of the DNA-imprinted gels was attributed to increasing cross-linking density by formation of duplex DNAs. Furthermore, the DNA-responsive swelling and shrinking behavior of the DNA-cross-linked gels and DNA-imprinted gels were strongly dependent upon sequences of DNAs when the gels were immersed in a buffer solution containing DNAs with various sequences. Therefore, the DNA-responsive gels have many potential applications as smart biomaterials for recognizing single nucleotide polymorphisms (SNPs)
Author keywords: DNA, gel, molecular imprinting, molecular recognition, SNPs, stimuli-responsive gel