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Abstract: Recognition phenomena of sugars on membrane surfaces are especially important because sugars are essential constituents in living bodies not only as energy sources and architecture-forming materials, but also as participants in many essential biological processes including recognition and response to outer signals. The phenylboronic acid (PBA) derivatives-carrying copolymer was accumulated on a colloidal gold-immobilized glass substrate, and the usefulness of the polymer brush as a sensing element for glycoproteins such as ovalbumin (OVA) was examined by using a UV-visible spectrophotometer with a help of localized surface plasmon resonance (LSPR). The sensor showed a concentration-dependent binding of OVA with a detection limit of 100 nM, and the association constants for the PBA group with glycoproteins were determined. Meanwhile, the sensor chip prepared by the simple incubation in a dithiolated PBA solution could not distinguish any glycoproteins from other ones specifically. Therefore, we tried to construct the sensor chip using the molecular imprinting technique, and succeeded in the construction of sensor chip which recognizes OVA selectively
Template and target information: protein, glycoprotein, ovalbumin, OVA
Author keywords: Au colloid, biosensor, Localized surface plasmon resonance technique