MIPdatabase
Authors: Ou JJ, Dong J, Tian TJ, Hu JW, Ye ML, Zou HF
Article Title: Enantioseparation of tetrahydropalmatine and Tröger's base by molecularly imprinted monolith in capillary electrochromatography.
Publication date: 2007
Journal: Journal of Biochemical and Biophysical Methods
Volume: 70
Issue: (1)
Page numbers: 71-76.
DOI: 10.1016/j.jbbm.2006.07.003
Alternative URL: http://www.sciencedirect.com/science/article/B6T28-4KKFXKK-1/2/5b47ab2fa4235b38f3f8c441bded9d12
Abstract: Two chiral compounds, Troger's base and tetrahydropalmatine, were enantioseparated on the (5S, 11S)-(-)-Tröger's base and l-tetrahydropalmatine imprinted monolithic capillary columns with CEC, respectively. The monoliths were prepared by in situ thermal-initiated copolymerization of methacrylic acid (MAA) and ethylene dimethacrylate (EDMA). After optimizing the ratio of porogens (toluene and dodecanol), the obtained monolithic capillary columns show good flow-through property and enantioselectivity. The influences of CEC parameters such as pH of the buffer, organic solvent and salt concentration on the electroosmotic flow (EOF) and recognition selectivity were systematically investigated. Under the optimal conditions, baseline resolutions of two chiral compounds were achieved. In addition, the fast separation was obtained within 4 min by applying higher voltage and assisting pressure of 6 bar
Template and target information: tetrahydropalmatine, (5S, 11S)-(-)-Tröger's base
Author keywords: Tetrahydropalmatine, Tröger's base, molecular imprinting polymer, capillary monolithic column, chiral separation, capillary electrochromatography
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