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Abstract: Emulsifer-free polymerization is used to prepare micron-size polystyrene. Naringenin molecularly imprinted polymeric microspheres (MIPMs) are prepared by single-step swelling polymerization using polystyrene as seeds. The influences of swelling ratios, ratios of water to oil, solvent and cross-linker dosage on morphology, size and size distribution of the MIPMs are investigated. Static adsorption is employed to evaluate the recognition properties of the MIPMs. When the ratio of water to oil is 7:1, and the swelling ratio is controlled in the range of 27~60, a series of uniformly sized MIPMs with particle diameter in the range of 4.5~6.0 μm can be obtained by single-step swelling polymerization using 1.5 μm polystyrene as seeds, cyclohexanol or chloroform as solvent, 20mmol ethylene glycol dimethacrylate as cross-linker. The prepared MIPMs exhibites a considerable tendency to adsorb naringenin and the printing molecules selectively, and the separation factor is up to 1.96 when quercetin is chosen as the competitive molecules.
Template and target information: naringenin
Author keywords: Single-step swelling polymerization, molecular imprinting, microsphere, naringenin