Abstract: Resveratrol,a promising anti-cancer agent, is an important active ingredent in Polygonum cuspidatum Sieb. et Zucc. A molecular imprinted polymer was prepared by virtue of molecular imprinting technique, with trans-resveratrol as the template molecular, acrylamide as the functional monomer, EGDMA as the cross-linking agent. The molecular imprinted polymer was investigated with the aid of equilibrium binding experiments to evaluate the molecular recognition and binding characteristics of the trans-resveratrol molecularly (imprinted) polymer. The scatchard analysis showed that two kinds of binding sites were produced in the polymer matrix and their dissociation constants were calculated to be 9.9×10~( -5) mol/L, 1.7×10~(-3) mol/L, respec-(tively). The results show that compared with non-imprinted polymer, the imprinted polymer exhibits a much higher affinity for trans-resveratrol among the tested compounds Res, Pol and BPA. Also, the imprinted (polymer) was applied to separate resveratrol from Polygonum cuspidatum Sieb. et Zucc. extracts and good (results) were obtained.
Template and target information: resveratrol
Author keywords: molecularly imprinted polymer, resveratrol, Polygonum cuspidatum Sieb.et Zucc., binding characteristics, substrate selectivity