MIPdatabase
Authors: Wang S, Wang YZ, Gong GQ, Chan XJ
Article Title: Studies on the molecular recognition and binding characteristics in rhodamine B molecularly imprinted polymer.
Publication date: 2003
Journal: Journal of Lanzhou University (Natural Sciences)
Volume: 39
Issue: (2)
Page numbers: 57-60.
Alternative URL: http://en.cnki.com.cn/Article_en/CJFDTotal-LDZK200302015.htm
Abstract: A molecularly imprinted polymer was prepared using acrylamide as monomer,rhodamine B as template molecule and a large amount of ethyleneglycol dimethacrylate as the cross- linking agent.The bulk polymer obtained was investigated in equilibrium binding experiments to evaluate the molecular recognition and binding characteristics of the rhodamine B molecularly imprinted polymer.Scatchard analysis showed thattwo sites were produced in the polymermatrix and theirdissociation constants were calculated to be1 .87× 1 0 -5mol/L and4.2 4× 1 0 -4mol/L respectively.The substrate selectivity of im- printed polymer and non- imprinted polymer was investigated.The results showed that the imprinted polymer exhibited much higher affinity for rhodamine B among the tested compounds.Finally,fluores- cence spectra indicted the interaction between the template and binding sites in the polymer.
Template and target information: rhodamine B
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