Abstract: This report describes preparation and characterization of ATP sensors based on Eelectrochemical quartz crystal microbalance (EQCM) electrode on which a layer of molecular imprinted polymer (MIP) was fabricated. Two serials of molecular imprinting polymer films, PPY/ATP and PPD/ATP, were prepared by electropolymerization of pyrrole and o-phenylenediamine in the presence of ATP following with electrochemical treatment to remove the temple molecules. Both of polypyrrole and poly(phenylenediamine) based EQCM sensors exhibit selective sensitivity towards ATP against ADP and AMP with different features. The effects of preparation and treatment conditions are investigated and optimized. PPD/ATP sensor was found to be superior both in sensitivity and response time with a detection limit at 5 x 10-6 M and a response time of about 50 s. The origin of the different molecular imprinting effect was also discussed.
Template and target information: ATP, adenosine triphosphate