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Abstract: The purpose of this study was to develop an HPLC procedure for determination of sulfamethazine (SMZ) and sulfachloropyridazine (SCP) on a molecularly imprinted polymeric (MIP) stationary phase. The MlPs were prepared by noncovalent polymerization. Sulfamethazine (SMZ), methacrylic acid (MAA), and ethylene glycoldimethacrylate (EGDMA) were used as template, functional monomer, and cross-linker, respectively, in the presence of chloroform as solvent. The MIPs obtained were packed into a stainless steel column (150 mm x 4.6 mm i.d.) and used as the stationary phase for HPLC. The mobile phase was 23 mm NaH2PO4 buffer solution-acetonitrile, 2:1 (v/v), at a flow-rate of 1 mL min(-1). The sample volume injected was 20 mu L and detection was by UV absorbance at 272 M. The retention times of SMZ and SCP under these conditions were approximately 7.015 +/- 0.182 min and 11.282 +/- 0.925 min. The total run time for each sample was 12 min. The selectivity of the MIPs was evaluated by analysis of different substances with molecular structures similar to that of SMZ. Separation factors (alpha) were used to compare chromatographic data from the stationary phases. The values of alpha obtained, in the range 1.57-1.72, showed that use of the MlPs enabled recognition of subtle structural differences from the template molecule. The limits of detection (LOD) of SMZ and SCP were 0.035 and 0.041 mu g L-1, respectively, and the respective limits of quantitation (LOQ) were 0.117 and 0.137 mu g L-1. The results showed that the SMZ-selective polymer was Suitable for separating both SMZ and SCP
Template and target information: sulfamethazine, SMZ, sulfachloropyridazine, SCP