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Reference type: Journal
Authors: Matsunaga T, Hishiya T, Takeuchi T
Article Title: Optimization of Functional Monomer Content in Protein-Imprinted Polymers.
Publication date: 2007
Journal: Analytical Letters
Volume: 40
Issue: (14)
Page numbers: 2633-2640.
DOI: 10.1080/00032710701588275
Alternative URL: http://www.informaworld.com/10.1080/00032710701588275

Abstract: We have prepared synthetic materials having binding specificity toward lysozyme using molecular imprinting. The lysozyme-imprinted polymers were prepared on silica beads using acrylic acid, acrylamide, and N,N'-methylenebisacrylamide. The molar ratio of acrylic acid to the template affected the selectivity and the maximum binding specificity was yielded with the molar ratio of 5 to 1 (acrylic acid/template). No binding specificity toward lysozyme was observed when there was no acrylic acid, and the binding specificity decreased when the amount of acrylic acid was high. The functional monomer-template ratio indicated in this study could be useful for improving the binding specificity in molecular imprinting of proteins
Template and target information: protein, lysozyme
Author keywords: molecular imprinting, lysozyme, acrylic acid, protein, N,N'-Methylenebisacrylamide


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