MIPdatabase
Authors: Teke M, Sezgintürk MK, Dinçkaya E, Telefoncu A
Article Title: Two Biosensors for Phenolic Compounds Based on Mushroom (Agaricus bisporus) Homogenate: Comparison in Terms of Some Important Parameters of the Biosensors.
Publication date: 2008
Journal: Preparative Biochemistry and Biotechnology
Volume: 38
Issue: (1)
Page numbers: 51-60.
DOI: 10.1080/10826060701774346
Alternative URL: http://www.informaworld.com/10.1080/10826060701774346
Abstract: Interest in molecular imprinted polymer techniques has increased because they allows for the improvement of some stability characteristics of enzymes. The high stability of molecularly imprinted enzymes for a substrate can make them ideal alternatives as recognition elements for sensors. A bioimprinted mushroom tissue homogenate biosensor was constructed in a very simple way. For this purpose, sulfite was used. The enzyme, polyphenol oxidase, was first complexed by using a competitive inhibitor, sulfite, in aqueous medium and then the enzyme was immobilized on gelatin by crosslinking with glutaraldehyde on a glass electrode surface. Similarly, polyphenol oxidase uncomplexed with sulfite was also immobilized on a glass electrode in the same conditions. The aim of the study was to compare the two biosensors in terms of their repeatability and thermal, pH, and operational stability; also, the linear ranges of the two biosensors were compared with each other
Template and target information: bioimprinting
Author keywords: Enzyme based biosensors, Polyphenol oxidase, Biosensor stability, Bioimprinted biosensors
Join the Society for Molecular Imprinting
New items RSS feed
View latest updatesSign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.
Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
