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Reference type: Journal
Authors: Zhao KY, Huang JJ, Ying XG, Cheng GX
Article Title: Macromolecularly imprinted calcium phosphate/alginate hybrid polymer microspheres with the surface imprinting of bovine serum albumin in inverse-phase suspension.
Publication date: 2008
Journal: Journal of Applied Polymer Science
Volume: 109
Issue: (4)
Page numbers: 2687-2693.
DOI: 10.1002/app.28354

Abstract: Macromolecularly imprinted calcium phosphate/alginate hybrid polymer microspheres surface imprinted with bovine serum albumin were prepared in inverse-phase suspension by introducing the intermixture solutions of bovine serum albumin (BSA) and CaCl2 in sodium alginate aqueous solution where (NH4)2HPO4 was added beforehand. Morphology of the imprinted microspheres was observed by optical microscopy. Rebinding dynamic and thermodynamic behaviors of surface molecularly imprinted calcium phosphate/alginate polymer microspheres (CP/A SMIPMs) and embedding molecularly imprinted calcium phosphate/alginate polymer microspheres (CP/A EMIPMs) were evaluated. CP/A SMIPMs exhibited significant improvement in equilibrium rebinding capacity (Qe) and imprinting efficiency (IE), compared to CP/A EMIPMs. The surface of CP/A SMIPMs was more competent to facilitate the migration and re-assembling of proteins. Surface specific peak and interior specific peak were proposed to describe the characteristics of surface and interior specific rebinding behaviors in rebinding dynamics. The effects of (NH4)2HPO4 addition were investigated in detail, along with the concentration of sodium alginate and CaCl2 solutions to the rebinding and imprinting property of imprinted microspheres. 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008
Template and target information: protein, bovine serum albumin, BSA
Author keywords: surface imprinting, calcium phosphate, alginate hybrid polymer microspheres, Bovine serum albumin, macromolecularly imprinted polymer microspheres, imprinting efficiency


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