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Reference type: Journal
Authors: Sadowska M, Wandelt B
Article Title: Molecularly Imprinted Thin Polymeric Film as a Fluorescent Sensor for Nucleotides.
Publication date: 2008
Journal: Molecular Crystals And Liquid Crystals
Volume: 486
Issue: (1)
Page numbers: 203-212.
DOI: 10.1080/15421400801918005

Abstract: The technology of molecular imprinting of polymers (MIPs) was used to obtain optical sensors for nucleotides. The methacrylates were used as functional monomers and 1,3-diphenyl-6-vinyl-1H-pyrazole-[3,4-b]-quinoline (PAQ) was used as a fluorescent receptor and the nucleotides: guanosine 3GǦ,5GǦ-cyclic monophosphate (cGMP) and adenosine 3GǦ,5GǦ-cyclic monophosphate (cAMP) were a template. The optical sensor obtained as a thin-layer polymeric films was investigated by steady-state fluorescence spectroscopy. The calculated association constant of the cGMP imprinted sensor is Ka-=-1.7-+-104-M-1 and of cAMP imprinted sensor Ka-=-9.9-+-104-M-1
Template and target information: guanosine 3',5'-cyclic monophosphate, cGMP, adenosine 3',5'-cyclic monophosphate, cAMP
Author keywords: fluorescence spectroscopy, MIPs, nucleotides, optical sensors, pyrazolequinolines

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