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Abstract: Molecular imprinting is a new approach to prepare predictable selectivity phase stationary and monolithic column is a novel technology for chromatographic stationary phase. The combination of monolith and molecularly imprinted polymers (MIPs) integrates the high efficiency of liquid chromatography and the high selectivity of MIPs, which will accelerate the develpoment of MIPs technique in the field of separation science. Therefore, the research in this field has attracted considerable interests and plays an important role in the area of analytical chemistry. In this review, the progress of molecularly imprinted monolith is summarized in detail from the polymerization conditions to prepare MIPs monolith, the separation conditions to evaluate the efficiency of MIPs, and the method of characterizing MIPs physical and chemical properties concerning the application of liquid chromatography-based MIPs. The limitations and prospective applications of the technique related with MIPs monolithic column are also discussed
Author keywords: in-situ polymerization, liquid chromatography, Molecularly imprinted polymers, monolithic columns