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Reference type: Journal
Authors: Diltemiz SE, Denizli A, Ersöz A, Say RI
Article Title: Molecularly imprinted ligand-exchange recognition assay of DNA by SPR system using guanosine and guanine recognition sites of DNA.
Publication date: 2008
Journal: Sensors and Actuators B: Chemical
Volume: 133
Issue: (2)
Page numbers: 484-488.
DOI: 10.1016/j.snb.2008.03.007
Alternative URL: http://www.sciencedirect.com/science/article/B6THH-4S32NKN-1/1/3eeda2c3bff4e93f6ab97f95df2bf0bb

Abstract: Molecular imprinting polymer (MIP) as a recognition element for sensors are increasingly of interest and MIP-SPR have started to appear in the literature. In this study, we have proposed a novel surface imprinting method for DNA recognition and determination by methacryl-amidohistidine-platinum(II) [MAH-Pt(II)] as a new metal-chelating monomer via metal coordination-chelation interactions and guanosine/guanine templates of DNA. The study includes the measurement of binding interaction of molecular imprinted surface plasmon resonance (SPR) sensor via ligand interaction and recognition selectivity studies of guanosine and guanine imprinted polymers with respect to guanine and dsDNA. Adsorption isotherms of adenosine, guanosine, guanine, adenine, ssDNA and dsDNA were plotted and affinity constants for guanosine and guanine imprinted films were also calculated
Template and target information: guanosine, guanine
Author keywords: Molecular imprinting polymers (MIPs), surface plasmon resonance (SPR), guanosine, Guanine, Ligand exchange (LE)


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