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Abstract: Molecularly imprinted films (MIF) were prepared for the detection of chloramphenicol succinate (CAP-SC) by photopolymerization method on screen-printed electrodes and superficial characteristic of these films was analyzed using electron microscope scanning. A sensor based on MIF for the detection of CAP-SC was assembled after the screen printed electrode was connected with an electrochemical analyzer through an electrode slot, and the detection result was recorded by the recorder connected with an electrochemical analyzer. Standard curve for CAP-SC detection was established and analysis of CAP-SC in milk samples was carried out. Scanning election microscopic images of the imprinted films showed that there were numerous imprinted micropores with a diameter about 100 nm on the surface of MIF. The MIF-based sensor showed high sensitivity and specificity toward the target CAP-SC and the response of MIF-sensor to concentration of CAP-SC displayed a linear correlation over a range from 1 Î 10-8 M to 1.2 Î 10-5 M with a detection limit of 2 Î 10-9 M. The recoveries reach 93.5%-95.5% based on milk samples
Template and target information: chloramphenicol succinate, CAP-SC
Author keywords: Molecularly imprinted films, Screen printed electrode, Chloramphenicol succinate, photopolymerization