MIPs logo MIPdatabase      Use this space
Custom Search
Reference type: Journal
Authors: Emgenbroich M, Borrelli C, Shinde S, Lazraq I, Vilela F, Hall AJ, Oxelbark J, De Lorenzi E, Courtois J, Simanova A, Verhage J, Irgum K, Karim K, Sellergren B
Article Title: A Phosphotyrosine-Imprinted Polymer Receptor for the Recognition of Tyrosine Phosphorylated Peptides.
Publication date: 2008
Journal: Chemistry - A European Journal
Volume: 14
Issue: (31)
Page numbers: 9516-9529.
DOI: 10.1002/chem.200801046

Abstract: Hyperphosphorylation at tyrosine is commonly observed in tumor proteomes and, hence, specific phosphoproteins or phosphopeptides could serve as markers useful for cancer diagnostics and therapeutics. The analysis of such targets is, however, a challenging task, because of their commonly low abundance and the lack of robust and effective preconcentration techniques. As a robust alternative to the commonly used immunoaffinity techniques that rely on phosphotyrosine(pTyr)-specific antibodies, we have developed an epitope-imprinting strategy that leads to a synthetic pTyr-selective imprinted polymer receptor. The binding site incorporates two monourea ligands placed by preorganization around a pTyr dianion template. The tight binding site displayed good binding affinities for the pTyr template, in the range of that observed for corresponding antibodies, and a clear preference for pTyr over phosphoserine (pSer). In further analogy to the antibodies, the imprinted polymer was capable of capturing short tyrosine phosphorylated peptides in the presence of an excess of their non-phosphorylated counterparts or peptides phosphorylated at serine
Template and target information: phosphotyrosine, pTyr
Author keywords: antibody mimics, epitome imprinting, molecular recognition, phosphopeptides, proteomics


  Shirt featuring the name Kal spelled out in symbols of the chemical elements  Mug featuring the name PeptideChemist spelled out in the single letter amino acid code  Chemists are fun customisable shirt

Molecules Special Issue call      Appeal for information






Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:
US listings   UK listings

Searching for Molecular Imprinting books on Amazon.com:
Molecular Imprinting
Imprinted Polymers
Polymer Chemistry
Organic Chemistry
Biosensors

Find what you need at Amazon.com

Lab supplies from Amazon