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Abstract: Penicillin G (PG) in milk is unsafe for human and is a big problem for the foods industry. Hence, the better analytical and eliminative techniques are demanded. We investigated a PG molecular imprinted polymer (MIP), which can sensitively detect and extract the PG from milk and other samples. The MIP synthesis involved a β-lactam antibiotics PG, methacrylic acid (MAA) and dimethyl formamide (DMF). Its properties were analyzed with Scatchard plot, which showed that there were two affinity sites of the PG polymer. The first equilibrium dissociation constant of the high-affinity binding sites Kd₁ was 1.14 x 10^-4 mol/L and the binding capacity Q_max1 was 46.01 μmol/g; the second equilibrium dissociation constant of the low-affinity binding sites Kd₂ was 1.35 x 10^-3 mol/L and the binding capacity Q_max2 was 72.55 μmol/g. Furthermore, two PG determination methods using the polymer were developed. The first was carried out quantitatively with a spectrophotometer and the detection limit was 1-áppm. The other one was the combination of the MIP particles with milk fermentation for PG analysis and the sensitivity was 10 ppb
Template and target information: penicillin G, beta-lactam antibiotic, PG
Author keywords: determination, extraction, milk, molecular imprinting polymer, penicillin