Abstract: Advances in molecular recognition have led to the rapid development concerning molecularly imprinted polymers (MIPs). The recognition specificity of MIPs towards template molecules, their stability, and low cost have led to much interest in using these polymers for the construction of lab-on-a-chip devices. Towards this end, we have developed a method to fabricate MIP microstructures using soft polydimethylsiloxane (PDMS) stamps containing relief microstructures. Using this method, microfilaments were obtained and characterized. Thin films of MIP were also generated between PDMS interfaces. We will present the fabrication of MIP thin films against 2,4-dichlorophenoxyacetic acid. Characterization of the thin films, as well as the equilibrium and competitive ligand binding studies will also be presented