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Abstract: The aim of the present work was to investigate the feasibility of employing the molecular imprinting polymer technique for detecting the mycotoxin zearalenone using a surface plasmon resonance (SPR) transducer. The molecularly imprinted polypyrrole (MIPPy) film was prepared by electropolymerization of pyrrole onto the bare Au chip in the presence of a template zearalenone molecule. The MIPPy-SPR sensor exhibited a linear response in the range of 0.3GêÆ3000 ng/mL (R(2) = 0.993) for detection of zearalenone. The selectivity efficiencies of zearalenone and other structurally related analogues were 1.0 and 0.15GêÆ0.27, respectively. The limit of detection and average recovery of blank corn matrix spiked with 30 ng/g zearalenone were 0.3 ng/g and 89%, respectively, and these were found to be comparable to those obtained by enzyme-linked immunosorbent assay. These results suggest that a combination of SPR sensing with MIPPy film is a promising alternative method for the detection of zearalenone
Template and target information: mycotoxin, zearalenone
Author keywords: zearalenone, polypyrrole, molecular imprinting polymer, surface plasmon resonance, enzyme-linked immunosorbent assay