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Reference type: Journal
Authors: Kempe M, Mosbach K
Article Title: Binding-studies on substrate-and enantio-selective molecularly imprinted polymers.
Publication date: 1991
Journal: Analytical Letters
Volume: 24
Issue: (7)
Page numbers: 1137-1145.
DOI: 10.1080/00032719108052959
Alternative URL: http://www.informaworld.com/10.1080/00032719108052959

Abstract: A molecularly imprinted polymer was prepared using tert- butyloxycarbonyl-L-phenylalanine as the print molecule and methacrylic acid as the functional monomer. The bulk polymer obtained was ground, sieved, packed into a column and investigated in the HPLC-mode by frontal chromatography to determine the number of binding sites and dissociation constants for the enantiomers interacting with the polymer. The dissociation constant for the L- enantiomer of the print molecule was lower than for the D-enantiomer (6.3 mM and 8.1 mM, respectively). This means that the affinity for the L-enantiomer was higher than for the D-enantiomer. The number of binding sites in the polymer giving rise to these dissociation constants were determined to be 28-mu-mol per g dry polymer
Template and target information: Boc-L-phenylalanine, tert-butyloxycarbonyl-L-phenylalanine

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