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Reference type: Journal
Authors: Zhou X, He XW, Chen LX, Li WY, Zhang YK
Article Title: Optimum Conditions of Separation Selectivity Based on Molecularly Imprinted Polymers of Bovine Serum Albumin Formed on Surface of Aminosilica.
Publication date: 2009
Journal: Chinese Journal of Analytical Chemistry
Volume: 37
Issue: (2)
Page numbers: 174-180.
DOI: 10.1016/S1872-2040(08)60084-5
Alternative URL: http://www.sciencedirect.com/science/article/B82YT-4VW50V8-3/2/74c9976bbcfcd4c2f5c9fb2e58ad3cb4

Abstract: Sol-gel molecularly imprinted polymers for recognition of bovine serum albumin were prepared via polymerization on the surface of aminopropyl silica followed by immobilization proteins. Based on the above system, the relationship between fundamental parameters of preparation of molecularly imprinted polymers and their effect on the separation selectivity was studied. A mixture of octyltrimethoxysilane, 3-aminopropylsilane, and tetraethoxysilane at 42.5:42.5:5:15 was polymerized at pH 7.0 before template protein removal by solution of 0.1 M NaOH and 10% (v/v) HAc-10% (w/v) SDS. The results indicated that the molecularly imprinted polymers obtained by this method showed the best separation selectivity. Moreover, the recognition mechanism of template proteins was analyzed preliminarily
Template and target information: protein, BSA, bovine serum albumin
Author keywords: surface imprinting, Separation selectivity, recognition mechanism, Bovine serum albumin


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