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Reference type: Journal
Authors: Kempe M, Glad M, Mosbach K
Article Title: An approach towards surface imprinting using the enzyme ribonuclease A.
Publication date: 1995
Journal: Journal of Molecular Recognition
Volume: 8
Issue: (1-2)
Page numbers: 35-39.
DOI: 10.1002/jmr.300080106

Abstract: An Adsorbent showing enhanced selectivity for the enzyme RNase A was prepared by a surface imprinting procedure based on metal coordination. A metal chelating monomer, N-(4-vinyl)-benzyl iminodiacetic acid, was polymerized onto methacrylate-derivated silica particles in the presence of RNase A and metal ions. Lysozyme and RNase A were separated on the adsorbent used as stationary phase in high-performance liquid chromatography.


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