Authors: Kempe M
, Glad M
, Mosbach K
An approach towards surface imprinting using the enzyme ribonuclease A.
Publication date: 1995
Journal: Journal of Molecular Recognition
Abstract: An Adsorbent showing enhanced selectivity for the enzyme RNase A was prepared by a surface imprinting procedure based on metal coordination. A metal chelating monomer, N-(4-vinyl)-benzyl iminodiacetic acid, was polymerized onto methacrylate-derivated silica particles in the presence of RNase A and metal ions. Lysozyme and RNase A were separated on the adsorbent used as stationary phase in high-performance liquid chromatography.
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