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Abstract: Macromolecularly imprinted calcium polyacrylate/alginate hybrid polymer microspheres with the imprinting of bovine serum albumin were prepared with sodium alginate and sodium polyacrylate by using CaCl2 as a gelling agent in inverse-phase suspension. Infrared spectrum and conductance titration demonstrated that hybrid components were produced in these microspheres. Rebinding isotherms of the microspheres proved a greater bovine serum albumin affinity for imprinted microspheres relative to nonimprinted ones. Selectivity tests indicated that calcium polyacrylate/alginate hybrid polymer microspheres exhibited good recognition properties for the template protein with the separation factor over 5.30 comparing to the competitive proteins with a similar Mw. The influence of cross-linker CaCl2 concentration on the imprinting efficiency (IE) and rebinding capacity was researched. It was found that the cross-linking density and imprinting efficiency of the hybrid hydrogel could be improved simultaneously. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009
Template and target information: protein, bovine serum albumin, BSA
Author keywords: calcium polyacrylate/ alginate (CPA/ A), Hybrid polymer microspheres, sodium alginate, sodium polyacrylate, Bovine serum albumin, molecular imprinting