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Reference type: Journal
Authors: Zhao KY, Wei JF, Cheng GX, Yang CX, Chen L
Article Title: Preparation of bovine serum albumin-imprinted calcium polyacrylate/alginate hybrid microspheres via Ca2+ crosslinking.
Publication date: 2009
Journal: Journal of Applied Polymer Science
Volume: 113
Issue: (2)
Page numbers: 1133-1140.
DOI: 10.1002/app.29936

Abstract: Macromolecularly imprinted calcium polyacrylate/alginate hybrid polymer microspheres with the imprinting of bovine serum albumin were prepared with sodium alginate and sodium polyacrylate by using CaCl2 as a gelling agent in inverse-phase suspension. Infrared spectrum and conductance titration demonstrated that hybrid components were produced in these microspheres. Rebinding isotherms of the microspheres proved a greater bovine serum albumin affinity for imprinted microspheres relative to nonimprinted ones. Selectivity tests indicated that calcium polyacrylate/alginate hybrid polymer microspheres exhibited good recognition properties for the template protein with the separation factor over 5.30 comparing to the competitive proteins with a similar Mw. The influence of cross-linker CaCl2 concentration on the imprinting efficiency (IE) and rebinding capacity was researched. It was found that the cross-linking density and imprinting efficiency of the hybrid hydrogel could be improved simultaneously. 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2009
Template and target information: protein, bovine serum albumin, BSA
Author keywords: calcium polyacrylate/ alginate (CPA/ A), Hybrid polymer microspheres, sodium alginate, sodium polyacrylate, Bovine serum albumin, molecular imprinting


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