MIPs logo MIPdatabase      Use this space
Custom Search
Reference type: Journal
Authors: Chuang SW, Rick J, Chou TC
Article Title: Electrochemical characterisation of a conductive polymer molecularly imprinted with an Amadori compound.
Publication date: 2009
Journal: Biosensors and Bioelectronics
Volume: 24
Issue: (10)
Page numbers: 3170-3173.
DOI: 10.1016/j.bios.2009.02.033

Abstract: Type II diabetes is a disease that is often characterised by elevated levels of advanced glycation end-products (AGEs), e.g. glycated haemoglobin (HbA1c), in a patient's bloodstream. This glycation reaction occurs when the carbonyl group of a circulating sugar (glucose) reacts with the amino group of the terminal valine residue of a haemoglobin chain, to form an unstable imine. This compound then undergoes an Amadori rearrangement to form the stable Amadori compound N-(1-deoxy-β-d-fructopyranose-1-yl)-l-valine (Fru-Val). As an initial approach to fabricating a sensor for the Fru-Val component of HbA1c, molecular imprints of Fru-Val were made in poly-aminophenylboronic acid (p-APBA), using ammonium persulphate as the initiator, on conductive indium-doped tin oxide (ITO) electrodes (nominal working area 0.5 cm2). The affinity of the imprints formed in the p-APBA, for fructose and valine as individual molecules, as well as for the complete template used for imprinting (Fru-Val), was assessed electrochemically, by the use of open circuit potential (Δ Eoc) measurements. These showed that the imprinted materials when challenged with Fru-Val had an open circuit response of ~5.0 x 10-3 V. d-Fructose (10 mM), a component of the template, when introduced into the cell gave a far more significant change in the open circuit potential (Δ Eoc = ~2.9 x 10-3 V) than did a similar concentration of d-glucose, a non-template carbohydrate (Δ Eoc = ~4.0 x 10-4 V). Non-template structured p-APBA films, made as controls in the absence of Fru-Val, showed negligible response to either d-fructose or d-glucose. Additionally, we have shown that the imprinted films show a progressive reduction in response to sequential additions of d-fructose, implying the saturation of imprinted sites and a limit to non-specific recognition
Template and target information: Amadori compound, N-(1-deoxy-β-d-fructopyranose-1-yl)-l-valine, Fru-Val
Author keywords: molecular imprinting, Maillard reaction, aminophenylboronic acid, Open circuit potential, Indium-doped tin oxide, Amadori compound


  mipdatabase.com logo imprinters do it in bulk shirt  Science Teacher peptide mug  Perpetual student shirt

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner