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Abstract: A molecularly imprinted HPLC monolithic column for enrofloxacin was prepared by an in situ thermal-initiated copolymerization technique for enrichment and rapid separation of a homologous series of fluoroquinolones. The morphological characteristics of the monolithic molecularly imprinted polymer (monolithic MIP) were investigated by scanning electron microscope, which showed that both mesopores and macropores were formed in the monolith, and the monolith allowed the mobile phase to flow through the column with low backpressure. Furthermore, some chromatographic conditions such as the influence of mobile phase composition and flow rate on the retention times were investigated. Hydrogen bonding interaction and hydrophobic interaction played important roles in the retention and separation. The binding capacity was evaluated by static adsorption and Scatchard analysis, which showed that the dissociation constant (KD ) and the maximum binding capacity (Qmax ) were 0.3275 mmol L-1, and 75.88 mmol g-1 for high affinity binding site, and 8.264 mmol L-1 and 521.5 mmol g-1 for lower affinity binding site, respectively. The present monolithic MIP was successfully applied to the quantitative determination of ciprofloxacin and enrofloxacin in eggs
Template and target information: ciprofloxacin, enrofloxacin, ENR, CIP
Author keywords: Enrofloxacin, food, molecularly imprinted polymer, monolithic column