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Reference type: Journal
Authors: Breton F, Delépée R, Agrofoglio LA
Article Title: Molecular imprinting of AMP by an ionic-noncovalent dual approach.
Publication date: 2009
Journal: Journal of Separation Science
Volume: 32
Issue: (19)
Page numbers: 3285-3291.
DOI: 10.1002/jssc.200900226

Abstract: In order to mimic recognition properties of adenylate kinase, molecularly imprinted polymers (MIPs) were prepared for adenosine 5prime-monophosphate (AMP), a substrate of the enzyme. Different functional monomers interacting with the phosphate moiety were tested, and the MIP giving the best specific binding of AMP was composed with one equivalent of 2-(dimethylamino)ethyl methacrylate and ten equivalents of acrylamide compared to AMP. Packed into solid phase cartridge, this polymer showed similar characteristics than the enzyme, since it was specific for AMP toward other nucleotides
Template and target information: adenosine 5'-monophosphate, AMP
Author keywords: Adenosine monophosphate, molecularly imprinted polymer, nucleotides, solid phase extraction


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