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Reference type: Journal
Authors: Liu XJ, Liu JZ, Huang YY, Zhao R, Liu GQ, Chen Y
Article Title: Determination of methotrexate in human serum by high-performance liquid chromatography combined with pseudo template molecularly imprinted polymer.
Publication date: 2009
Journal: Journal of Chromatography A
Volume: 1216
Issue: (44)
Page numbers: 7533-7538.
DOI: 10.1016/j.chroma.2009.06.018
Alternative URL: http://www.sciencedirect.com/science/article/B6TG8-4WGVPT5-2/2/875aa6fae75fffacc39d9576f33d5273

Abstract: A pseudo template molecularly imprinted polymer (MIP) was prepared for methotrexate (MTX) and a RP-HPLC method combined with the MIP was developed for the determination of MTX in human serum. Because of the poor solubility of MTX in common MIP preparation solvents, trimethoprim (TMP), a molecule having the similar imprinting sites as MTX, is selected as the pseudo template. The MIP was prepared using methacrylic acid (MAA) and ethylene glycol dimethacrylate as functional monomer and cross-linker, respectively. 1H NMR study showed highly strong interaction between TMP and MAA with hydrogen bonds. Chromatographic behaviors indicated that the TMP-MIP possessed excellent affinity and selectivity for MTX. And the imprinting factor for MTX was high up to 9.5 when 7:3 of acetonitrile:methanol (v/v) was used as mobile phase. Moreover, TMP-MIP was used as the solid-phase extraction (SPE) material to enrich the target compound MTX in human serum samples for HPLC analysis. The SPE process was carefully optimized and good recoveries of MTX were obtained as 81.6-86.2% with RSD of 0.22-1.84% when the spiked concentration of MTX was 2.0-10.0áμgámL-1 in human serum samples. The results indicated that the pseudo template MIP can be applied to preconcentration, purification and analysis of MTX in clinic samples
Template and target information: trimethoprim, TMP, methotrexate, MTX
Author keywords: molecularly imprinted polymer, Pseudo template, Methotrexate, Solid-phase extraction, Human serum analysis

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