MIPs logo MIPdatabase      Use this space
Custom Search
Reference type: Journal
Authors: Pasetto P, Flavin K, Resmini M
Article Title: Simple spectroscopic method for titration of binding sites in molecularly imprinted nanogels with hydrolase activity.
Publication date: 2009
Journal: Biosensors and Bioelectronics
Volume: 25
Issue: (3)
Page numbers: 572-578.
DOI: 10.1016/j.bios.2009.03.042
Alternative URL: http://www.sciencedirect.com/science/article/B6TFC-4W0R0JJ-1/2/dfdacd193f20a12c2689a0eb5193c4ca

Abstract: In this investigation we report the preparation of soluble molecularly imprinted catalytic nanogels with hydrolytic activity. The nanogels were imprinted using a stoichiometric non-covalent approach, employing a phosphate transition state analogue as template and polymerizable tyrosine and arginine units as functional monomers, for catalysis of a carbonate hydrolysis reaction. Full characterization of the rebinding and of the hydrolytic activity was performed, with particular emphasis on a novel titration method developed for the measurement of active site concentrations and the subsequent calculation of accurate catalytic parameters. Considering the features of the template molecule and the functional monomers used, an original method for performing rebinding experiments is described, taking advantage of the change of the visible spectrum evident on binding the sodium salt of the template to the arginine residue present in the nanogel
Template and target information: phosphate transition state analogue, TSA, 4-nitro-4'-acetamino-diphenylphosphate, 4-nitro-4'-acetamino-diphenylcarbonate
Author keywords: Molecularly imprinted polymers, molecular imprinting, Nanogels, catalysis, Recognition, Active site titration, Enzyme mimic


  MIPs are Forever spoof movie poster mug  Mug featuring the name Pamela spelled out in the single letter amino acid code  Chemists are fun customisable shirt

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner