Abstract: The instability of enzymatic glucose sensors has prevented the development of a practical artificial pancreas for diabetic patients. We therefore developed an enzyme-free glucose sensor using the gate effect of the molecularly imprinted polymer (MIP). This sensor has the advantage of improved stability and reduced procedure. An adduct of glucose and 4-vinylphenylboronic acid (VPBA) was synthesized by esterification and was then purified. The copolymer of the glucose/VPBA adduct and methylene bisacrylamide was grafted onto an indium tin oxide electrode surface. Glucose was washed out from the copolymer to obtain an MIP layer. Cyclic voltammetry of ferrocyanide in aqueous solution was performed using an MIP-grafted electrode, and the effect of glucose on the anodic current intensity was evaluated. The anodic current intensity was sensitive to the glucose concentration, and the dynamic range (0–900 mg/dL) covered the typical range of diabetic blood glucose levels. The response time of the MIP-grafted electrode to the stepwise change of the glucose concentration is approximately 3 to 5 min. Thus, we can conclude that the MIP-grafted electrode is feasible as a glucose sensor for monitoring blood sugar in diabetic patients using its gate effect.