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Abstract: Aim: To prepare phenylalanine molecularly imprinted polymers (Phe-MIPs) on a porous silica-gel support in aqueous phase and study on its chromatography. Methods: By copolymerizing acryloyl β-cyclodextrin, with 2-acrylamido-2-methyl-1-propane-sulfonic acid (AMPSA) as the functional monomer, and N, N'-methylene bisacrylamide (MBAA) as the cross-linker on the vinylated silica-gel in aqueous phase of phenylalanine, a thin layer of molecularly imprinted β-cyclodextrin (β-CD) polymer was immobilized on silica-gel via thermal polymerization. The prepared Phe-MIPs were packed into an empty stainless steel column. HPLC-UV was performed on this column with a mobile phase of MeCN-H2O-HCOOH (10: 90: 0.1) at a flow rate of 0.1 mL/min and a detection wavelength of 200 nm. The Phe-MIPs column were compared with the blank column. Results: Phenylalanine had stronger retention on Phe-MIPs column than the blank imprinted polymers column, and was well separated with lysine and proline. Conclusion: The MIPs prepared in aqueous medium have a strong adsorption selectivity
Template and target information: phenylalanine, Phe
Author keywords: HPLC, molecular imprint, silica gel, Surface polymerization, Thermal polymerization