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Publisher: SPIE
Volume number: 7493
Conference information: Proceedings of SPIE - The International Society for Optical Engineering

Abstract: Molecularly imprinted polymers (MIPs) with excellent molecular recognition ability have been used in chemical sensors, chromatographic separation and biochemical analyses. Thymidine is an important part of DNA for biomolecular recognition and the intermediate of many medicines. The polymers imprinted with the template of thymidine and 5GǦ-Otosylthymidine have been prepared, using a non-proton solvent, acetonitrile as the porogen. Direct imprinting with thymidine could not form strong molecular interaction sites in this system. Relative MIPs were obtained by bulk polymerization and their adsorption capacities were investigated. The adsorption capacities of MIP (P2) and nonimprinted polymer (P20) for thymidine are 0.120 mg-Àg-1and 0.103 mg-Àg-1, respectively. The imprinting factor is 1.17. As 5GǦ-O-tosylthymidine is more soluble than thymidine moiety in acetonitrile and give rise to more sites of molecular recognition. The results demonstrated that the imprinted polymers were able to bind and recognize thymidine moderately in acetonitrile. MIPs imprinted with 5GǦ-O-tosylthymidine like nature enzymes displayed some recognition ability to its analogues. The insoluble derivatives in the non-proton solvent can be an effective template to prepare efficient imprinting recognition sites. -® 2009 Copyright SPIE - The International Society for Optical Engineering
Template and target information: thymidine, 5'-O-tosylthymidine
Author keywords: column separation, molecular recognition, Molecularly imprinted polymers, Thymidine