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Abstract: A new molecularly imprinted polymer (MIP)-chemiluminescence (CL) detection approach towards recognition of dansyl-glycine (Gly) is presented. The polymer microspheres were synthesized using precipitation polymerization with glycine as template and immobilized in microtiter plates (96 wells) using poly(vinyl alcohol) (PVA) as glue. The analyte (dansyl-glycine) was selectively adsorbed on the MIP microspheres. After washing, the bound fraction was quantified based on a dansyl-glycine-bis(2,4,6-trichlorophenyl) oxalate (TCPO)-H2O2 chemiluminescence reaction enhanced by imidazole. The chemiluminescence intensity has a linear relationship with the concentration of glycine in the range of 0.2 similar to 60 mu mol/L, with the limit of detection (LOD) being 0.07 mu mol/L (3 sigma). The relative standard deviation (RSD) for 11 parallel measurements of glycine (1 mu mol/L) was 3.3%. The cavity of MIP synthesized with glycine as template is smaller, which can avoid non-specific adsorption and lead to enhancement of specificity, response speed and sensitivity when recognizing dansyl-glycine
Template and target information: dansyl glycine, glycine
Author keywords: molecularly imprinted polymer, chemiluminescence, array sensor, glycine