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Reference type: Journal
Authors: Claude B, Viron-Lamy C, Haupt K, Morin P
Article Title: Synthesis of a molecularly imprinted polymer for the solid-phase extraction of betulin and betulinic acid from plane bark.
Publication date: 2010
Journal: Phytochemical Analysis
Volume: 21
Issue: (2)
Page numbers: 180-185.
DOI: 10.1002/pca.1175

Abstract: Introduction - Plant extracts are usually complex mixtures of various polarity compounds and their study often includes a purification step, such as solid-phase extraction (SPE), to isolate interest compounds prior analytical investigations. Molecularly imprinted polymers (MIPs) are a new promising type of SPE material which offer tailor-made selectivity for the extraction of trace active components in complex matrices. Numerous specific cavities that are sterically and chemically complementary of the target molecules, are formed in imprinted polymers. A molecularly imprinted polymer (MIP) was synthesised in order to trap a specific class of triterpene, including betulin and betulinic acid from a methanolic extract of plane bark.Methodology - Imprinted polymers were synthesised by thermal polymerisation of betulin as template, methacrylic acid (MAA) or acrylamide (AA) as functional monomer, ethylene glycol dimethacrylate as crosslinking agent and chloroform as porogen. Afterwards, MAA- and AA-MIPs were compared with their non-imprinted polymers (NIPs) in order to assess the selectivity vs betulin and its derivatives. Recovered triterpenes were analysed by HPLC during MIP-SPE protocol.Results - After SPE optimisation, the MAA-imprinted polymer exhibited highest selectivity and recovery (better than 70%) for betulin and best affinity for its structural analogues. Thus, a selective washing step (chloroform, acetonitrile) removed unwanted matrix compounds (fatty acids) from the SPE cartridge. The elution solvent was methanol. Finally, the MAA-MIP was applied to fractionate a plane bark methanolic extract containing betulin and betulinic acid.Conclusion - This study demonstrated the possibility of direct extraction of betulin and its structural analogues from plant extracts by MIP technology. Copyright 2009 John Wiley & Sons, Ltd
Template and target information: betulin, betulinic acid
Author keywords: molecularly imprinted polymer, MIP, Solid-phase extraction, SPE, betulin, betulinic acid, Triterpene, plane bark extract


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